Overview

The UCSF ES Cell Targeting Core is set to provide high-quality ES cell services with a high probability of germline transmission. The core provides ES cell targeting, genomic DNA extraction from 96-well plates, expansion of targeted ES cells, mycoplasma test, chromosome counts, and preparation of ES cells for microinjection, to all UCSF investigators as well as external investigators.

Prior to initiation of a project, consultation is available from the director on the entire procedures of generating knockout mice. This meeting will outline specifics of the project including knockout strategy, KO construct design, screening assays, and other issues relevant to microinjection and chimera breeding.

We work with the Gladstone Transgenic Gene Targeting Core for your microinjections to deliver a full-range gene targeting service. On the day of injection, we prepare cells and deliver them to Gladstone.

Guidelines For Getting ES Clones From Repositories and Making Mice

NEW SERVICE! TALEN service now available to UCSF investigators!

Scheduling and Requests

Investigators request services by submitting a Service Requisition Form (by email or in person). A university-wide online submission system will be in place soon. Services will be performed in the order that requests are received. Please provide all the information that is required. Until all the information is submitted, your project will not be scheduled on our calendar.

Contact:
Genentech Hall S472E
600 16th Street
San Francisco, CA 94158
Campus mailbox: 2202
Phone: 415-514-1219
Email: ESCore@ucsf.edu

For more information on core facilities at UCSF, please visit the Research Resource Program (RRP), or to find instruments or services at UCSF, visit the new Cores Search.

Note: If the work done with this Core produces data resulting in a figure in a publication, you are required to acknowledge the Core ("UCSF ES Cell Targeting Core") in the publication.