Overview

The UCSF ES Cell Targeting Core is set to provide high-quality ES cell services with a high probability of germline transmission. The core provides ES cell targeting, genomic DNA extraction from 96-well plates, expansion of targeted ES cells, mycoplasma test, chromosome counts, and preparation of ES cells for microinjection, to all UCSF investigators as well as external investigators.

Prior to initiation of a project, consultation is available from the director on the entire procedures of generating knockout mice. This meeting will outline specifics of the project including knockout strategy, KO construct design, screening assays, and other issues relevant to microinjection and chimera breeding.

Guidelines For Getting ES Clones From Repositories and Making Mice

Scheduling and Requests

Investigators request services by submitting a Service Requisition Form online (or by email). Services will be performed in the order that requests are received. Please provide all the information that is required. Until all the information is submitted, your project will not be scheduled on our calendar.

Contact:
Genentech Hall S472
600 16th Street
San Francisco, CA 94158
Campus mail code: 0407
Phone: 415-514-1219
Fax: 415-476-2721

For more information on core facilities at UCSF, please visit the Research Resource Program (RRP).